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cell protein concentrations  (Bio-Rad)


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    Bio-Rad cell protein concentrations
    Cell Protein Concentrations, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 16567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 16567 article reviews
    cell protein concentrations - by Bioz Stars, 2026-05
    97/100 stars

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    Hek-WNT11 sEVs induce GSK3β phosphorylation. ( A ) NTA distribution plot of the hydrodynamic diameter size of Hek-WNT11 sEVs. ( B ) The Western blot analysis of the sEVs secreted by wild type and WNT11-HA tag-overexpressing HEK <t>cells</t> shows the presence of the well-established exosomal marker CD63, and the WNT11 or HA tag (10 µg of total proteins were loaded per lane). Molecular weight markers (kDa) are indicated. ( C ) Western blot analysis of GSK3α/β, JNK and β-catenin in C2C12 myotubes treated with increasing <t>concentrations</t> of Hek sEVs (lanes 2–4) or Hek-WNT11 sEVs (lanes 5–7) as indicated in the figure. Non-treated C2C12 myotubes represent the control (lane 1); Tubulin was used as the housekeeping <t>protein.</t> Molecular weight markers (kDa) are indicated. Original, uncropped immunoblots are reported in Additional File - Fig. . ( D ) Expression of GSK3 inactive form e JNK active form was measured as ratio of the phosphorylated isoform/not phosphorylated isoform. The fold change refers to the control condition. *** p < 0.001, one-way ANOVA test followed by Dunnett’s post-hoc test
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    Hek-WNT11 sEVs induce GSK3β phosphorylation. ( A ) NTA distribution plot of the hydrodynamic diameter size of Hek-WNT11 sEVs. ( B ) The Western blot analysis of the sEVs secreted by wild type and WNT11-HA tag-overexpressing HEK cells shows the presence of the well-established exosomal marker CD63, and the WNT11 or HA tag (10 µg of total proteins were loaded per lane). Molecular weight markers (kDa) are indicated. ( C ) Western blot analysis of GSK3α/β, JNK and β-catenin in C2C12 myotubes treated with increasing concentrations of Hek sEVs (lanes 2–4) or Hek-WNT11 sEVs (lanes 5–7) as indicated in the figure. Non-treated C2C12 myotubes represent the control (lane 1); Tubulin was used as the housekeeping protein. Molecular weight markers (kDa) are indicated. Original, uncropped immunoblots are reported in Additional File - Fig. . ( D ) Expression of GSK3 inactive form e JNK active form was measured as ratio of the phosphorylated isoform/not phosphorylated isoform. The fold change refers to the control condition. *** p < 0.001, one-way ANOVA test followed by Dunnett’s post-hoc test

    Journal: Cell Communication and Signaling : CCS

    Article Title: Synergic action of MicroRNAs and Wnts delivered by motor neuron EVs in promoting AChR clustering

    doi: 10.1186/s12964-025-02312-x

    Figure Lengend Snippet: Hek-WNT11 sEVs induce GSK3β phosphorylation. ( A ) NTA distribution plot of the hydrodynamic diameter size of Hek-WNT11 sEVs. ( B ) The Western blot analysis of the sEVs secreted by wild type and WNT11-HA tag-overexpressing HEK cells shows the presence of the well-established exosomal marker CD63, and the WNT11 or HA tag (10 µg of total proteins were loaded per lane). Molecular weight markers (kDa) are indicated. ( C ) Western blot analysis of GSK3α/β, JNK and β-catenin in C2C12 myotubes treated with increasing concentrations of Hek sEVs (lanes 2–4) or Hek-WNT11 sEVs (lanes 5–7) as indicated in the figure. Non-treated C2C12 myotubes represent the control (lane 1); Tubulin was used as the housekeeping protein. Molecular weight markers (kDa) are indicated. Original, uncropped immunoblots are reported in Additional File - Fig. . ( D ) Expression of GSK3 inactive form e JNK active form was measured as ratio of the phosphorylated isoform/not phosphorylated isoform. The fold change refers to the control condition. *** p < 0.001, one-way ANOVA test followed by Dunnett’s post-hoc test

    Article Snippet: The cell body protein concentration was determined by the Bradford assay using Bio-Rad Protein Assay Dye Reagent Concentrate (BioRad).

    Techniques: Phospho-proteomics, Western Blot, Marker, Molecular Weight, Control, Expressing